Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)
![Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)](https://www.abcam.com/ps/products/248/ab248641/Images/ab248641-368082-anti-clptm1-antibody-epr8800-western-blot-knockout-hap1-clptm1-ko-a549-hela-human-lysates.jpg "Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8800] to CLPTM1 - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free
See all CLPTM1 primary antibodies -
Description
Rabbit monoclonal [EPR8800] to CLPTM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, A549 and HeLa cell lysates.
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General notes
ab248641 is the carrier-free version of ab133756 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248641 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 3.45 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8800 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLPTM1 knockout HAP1 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76 kDa
Observed band size: 76 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133756 observed at 76 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133756 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in CLPTM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CLPTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab133756 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133756).
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![Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)](https://www.abcam.com/ps/products/248/ab248641/Images/ab248641-2-benefits-of-recombinant-antibodies.png)
Anti-CLPTM1 antibody [EPR8800] - BSA and Azide free (ab248641)