Anti-SARA antibody [EPR5020] (ab124875)
![Anti-SARA antibody [EPR5020] (ab124875)](https://www.abcam.com/ps/products/124/ab124875/Images/ab124875-138805-anti-sara-antibody-epr5020-western-blot.jpg "Anti-SARA antibody [EPR5020] (ab124875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5020] to SARA
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SARA antibody [EPR5020]
See all SARA primary antibodies -
Description
Rabbit monoclonal [EPR5020] to SARA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human SARA aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Huan heart, A673, HT-1080, BxPC-3, Jutkat, HeLa, and Human fetal brain lysates, Human kidney tissue
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 3.10 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5020 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARA antibody [EPR5020] (ab124875)All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Human heart lysate
Lane 2 : A673 cell lysate
Lane 3 : HT-1080 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDa
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![Western blot - Anti-SARA antibody [EPR5020] (ab124875)](https://www.abcam.com/ps/products/124/ab124875/Images/ab124875-138804-anti-sara-antibody-epr5020-western-blot.jpg)
Western blot - Anti-SARA antibody [EPR5020] (ab124875)All lanes : Anti-SARA antibody [EPR5020] (ab124875) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 156 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] (ab124875)](https://www.abcam.com/ps/products/124/ab124875/Images/ab124875-138803-anti-sara-antibody-epr5020-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020] (ab124875)Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SARA antibody [EPR5020] (ab124875)](https://www.abcam.com/ps/products/124/ab124875/Images/ab124875-138802-anti-sara-antibody-epr5020.jpg)
OI-RD Scanning - Anti-SARA antibody [EPR5020] (ab124875) -
![Anti-SARA antibody [EPR5020] (ab124875)](https://www.abcam.com/ps/products/124/ab124875/Images/ab124875-5-benefits-of-recombinant-antibodies.png)
Anti-SARA antibody [EPR5020] (ab124875)
