Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18104] to PKC alpha + beta 2 + gamma - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free
    See all PKC alpha + beta 2 + gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR18104] to PKC alpha + beta 2 + gamma - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250778 is the carrier-free version of ab184746. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250778 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    All lanes : Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC gamma full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77, 78 kDa


    Exposure time: 1 second


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)

    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PKC gamma with ab184746 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasmic staining on Human pancreatic acinar cells is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunocytochemistry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)

    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling PKC gamma with ab184746 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab184746 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Flow Cytometry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Flow Cytometry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)

    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PKC gamma with ab184746 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    All lanes : Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution

    Lane 1 : Active human PKC beta 2 full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC delta full length protein
    Lane 4 : Active human PKC eta full length protein
    Lane 5 : Active human PKC epsilon full length protein
    Lane 6 : Active human PKC theta full length protein
    Lane 7 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77, 78 kDa


    Exposure time: 3 minutes


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution + Active human PKC beta 1 full length protein at 0.02 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77, 78 kDa


    Exposure time: 3 minutes


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.

  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 77, 78 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    All lanes : Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 77, 78 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Western blot - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    All lanes : Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] (ab184746) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 77, 78 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab184746, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunocytochemistry - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    This data was developed using ab184746, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling PKC gamma with ab184746 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic and nuclear staining on HeLa cell line.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab184746 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    This data was developed using ab184746, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded cervical cancer tissue labeling PKC gamma with ab184746 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasmic staining on cancer cells of cervix cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    This data was developed using ab184746, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling PKC gamma with ab184746 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on epithelial cells of mouse kidney is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    This data was developed using ab184746, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PKC gamma with ab184746 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasmic staining on epithelial cells of rat kidney is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    This data was developed using ab184746, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling PKC gamma with ab184746 at 1/400 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Weak cytoplasmic staining on neuron of Human cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)
    Anti-PKC alpha + beta 2 + gamma antibody [EPR18104] - BSA and Azide free (ab250778)

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