Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-THBS4 antibody [EPR22922-232] (ab263898)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22922-232] to THBS4
Suitable for: WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-THBS4 antibody [EPR22922-232]
See all THBS4 primary antibodies
Description

Rabbit monoclonal [EPR22922-232] to THBS4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Rat
ICC/IF	Mouse Rat
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle, human serum, mouse skeletal muscle, human articular cartilage, mouse kidney, mouse articular cartilage, rat heart and rat articular cartilage lysates. ICC/IF: C2C12 and L6 cells. Flow Cyt: C2C12 and L6 cells. IP: Human and mouse skeletal muscle whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22922-232
Isotype

IgG
Research areas

Western blot - Anti-THBS4 antibody [EPR22922-232] (ab263898)

All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution

Lane 1 : Human articular cartilage lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse articular cartilage lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat articular cartilage lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 106 kDa

The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).

Exposure times: Lanes 1-4: 15 seconds; Lane 5: 6 seconds.
Western blot - Anti-THBS4 antibody [EPR22922-232] (ab263898)

All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human serum lysate
Lane 3 : Mouse skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 106 kDa
Observed band size: 100,120,140,75 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).

Exposure times: Lane 1: 48 secs; Lane 2: 6 secs; Lane 3: 1 sec.
Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] (ab263898)

THBS4 was immunoprecipitated from 0.35 mg mouse skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Mouse skeletal muscle lysate 10µg

Lane 2: ab263898 IP in mouse skeletal muscle lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in mouse skeletal muscle lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] (ab263898)

THBS4 was immunoprecipitated from 0.35 mg human skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Human skeletal muscle lysate 10µg

Lane 2: ab263898 IP in human skeletal muscle lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in human skeletal muscle lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] (ab263898)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] (ab263898)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] (ab263898)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] (ab263898)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-THBS4 antibody [EPR22922-232] (ab263898)

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