Anti-THBS4 antibody [EPR22922-232] (ab263898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22922-232] to THBS4
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-THBS4 antibody [EPR22922-232]
See all THBS4 primary antibodies -
Description
Rabbit monoclonal [EPR22922-232] to THBS4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseRatICC/IF MouseRatIP MouseHumanWB MouseRatHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle, human serum, mouse skeletal muscle, human articular cartilage, mouse kidney, mouse articular cartilage, rat heart and rat articular cartilage lysates. ICC/IF: C2C12 and L6 cells. Flow Cyt: C2C12 and L6 cells. IP: Human and mouse skeletal muscle whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22922-232 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse articular cartilage lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 106 kDaThe expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).
Exposure times: Lanes 1-4: 15 seconds; Lane 5: 6 seconds.
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All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human serum lysate
Lane 3 : Mouse skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 100,120,140,75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).
Exposure times: Lane 1: 48 secs; Lane 2: 6 secs; Lane 3: 1 sec.
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THBS4 was immunoprecipitated from 0.35 mg mouse skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse skeletal muscle lysate 10µg
Lane 2: ab263898 IP in mouse skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in mouse skeletal muscle lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
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THBS4 was immunoprecipitated from 0.35 mg human skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Human skeletal muscle lysate 10µg
Lane 2: ab263898 IP in human skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in human skeletal muscle lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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