Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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ELISA - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)
Immobilized ab221235 at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag with a linear range of 1.56-12.5 ng/mL.
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Functional Studies - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)The purity of Human SIRP alpha, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC. -
Functional Studies - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)FACS assay shows that recombinant Human SIRP alpha, Fc Tag (HPLC-verified) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 µg/ml. -
SDS-PAGE - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)Human SIRP alpha, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. -
SDS-PAGE - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221235)SDS-PAGE analysis of ab221235 under reducing conditions, stained overnight with Coomassie Blue. As a result of glycosylation, the reduced protein migrates as 70-105 kDa.
