Recombinant Human SIRP alpha protein (ab174006)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
Images
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SDS-PAGE - Recombinant Human SIRP alpha protein (ab174006)
Reduced SDS-PAGE analysis of ab174006 staining overnight with Coomassie Blue.
DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)FACS analysis shows that the binding of Human SIRP alpha, His Tag (HPLC-verified) (ab174006) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.2257 μg/mL
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)FACS assay shows that Human SIRP alpha, His Tag (HPLC-verified) (ab174006) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Immobilized Human CD47, Fc Tag (HPLC-verified) (ab220587) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 0.72 μM as determined in BLI assay
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin (ab220559) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay
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Biological activity - Recombinant Human SIRP alpha protein (ab174006)Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) ab174006, Biotinylated Human CD47, Fc,Avitag ab246018, binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL
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HPLC - Recombinant Human SIRP alpha protein (ab174006)The purity of Human SIRP alpha, His Tag (HPLC-verified) ab174006 was greater than 90% as determined by SEC-HPLC.
