Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Tenascin C antibody [EPR4219] (ab108930)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4219] to Tenascin C
Suitable for: IHC-P, WB, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-Tenascin C antibody [EPR4219]
See all Tenascin C primary antibodies
Description

Rabbit monoclonal [EPR4219] to Tenascin C
Host species

Rabbit
Specificity

IHC on human tissues which we tested (such as testis, pancreas and stomach) showed non-specific staining. We don’t recommend this antibody for IHC on human tissues.

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-Fr: Mouse E14 spinal cord and cerebellar cortex tissue; Rat cerebellar cortex tissue. IHC-P: Rat cerebellar cortex tissue; Mouse E14 spinal cord and cerebellar cortex tissue. WB: U87-MG cell lysate; Postnatal mouse cerebellum lysate; Postnatal rat brain lysate; Human fetal brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4219
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA Buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain. Staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Frozen sections) analysis of mouse E14 spinal cord labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

Lane 1 : Human fetal brain
Lane 2 : Human liver

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 241 kDa
Observed band size: 250 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM /TBST.

Liver is negative control (PMID: 1717349). The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14 spinal cord tissue sections labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Hematoxylin counterstain.

Positive staining on mesenchymal condensations during chondrogenesis of mouse E14 embryo (PMID: 9822997; PMID: 19586317; PMID: 24778247) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + U87-MG (human glioblastoma) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 241 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM /TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Frozen sections) analysis of rat cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on the molecular layer of rat cerebellar cortex (PMID: 1372043) is observed.
Immunohistochemistry (Frozen sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Frozen sections) analysis of mouse E14 cerebellar cortex labeling Tenascin C with ab108930 at 1/100 dilution (4.27μg/ml). Tissue was fixed with 4% PFA and permeabilized with 0.2% TritonX-100. Antigen retrieval was performed using a heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). ab150077, an AlexaFluor^®488 Goat anti-Rabbit secondary antibody was used at 1/1000 (2 μg/ml). DAPI nuclear counterstain.

Positive staining on the molecular layer of mouse E14 cerebellar cortex (PMID: 1372043) is observed.
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

All lanes : Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution

Lane 1 : Postnatal (P0) mouse cerebellum
Lane 2 : Postnatal (P0) rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 241 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure Time:
Lane 1: 15 seconds
Lane 2: 30 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 10462531).
Western blot - Anti-Tenascin C antibody [EPR4219] (ab108930)

Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

Predicted band size: 241 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [EPR4219] (ab108930)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellar cortex labeling Tenascin C with ab108930 at 1/500 dilution (0.854 μg/ml). Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9 (ab93684). Hematoxylin was used to counterstain. A ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used. Staining on the molecular layer of mouse cerebellar cortex (PMID: 1372043) is observed.
Anti-Tenascin C antibody [EPR4219] (ab108930)

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