Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Flow Cyt, ELISA, SDS-PAGE, HPLC, Functional Studies
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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ELISA - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)
Recombinant human CD47 protein (Active) (ab174029) at 2 µg/mL (100 µL/well) can bind ab221342 with a linear range of 4-31 ng/mL.
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SDS-PAGE - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)SDS PAGE of reduced ab221342 stained overnight with Coomassie Blue. The protein migrates as 70-105 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)The purity of ab221342 was greater than 90% as determined by SEC-HPLC.
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ELISA - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into ab221342: Recombinant human CD47 protein (Fc Chimera Active) (Biotin) (ab246018) binding reactions. The half maximal inhibitory concentration (IC50) is 0.2006 µg/mL.
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Flow Cytometry - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)FACS assay shows that recombinant ab221342 can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 0.3 µg/ml.
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Functional Studies - Recombinant human SIRP alpha protein (Fc Chimera Active) (ab221342)FACS analysis shows that the binding of ab221342 to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing anti-CD47 antibody. The concentration of SIRP alpha used is 0.3 µg/ml. IC50=0.1318 µg/ml.
