Human H2AFY (mH2A1) knockout HEK-293T cell lysate (ab257463)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260244 - Human H2AFY knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human H2AFY (mH2A1) knockout HEK293T cell lysate (ab257463)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: H2AFY knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab183041 observed at 40 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab183041 Anti-mH2A1 antibody [EPR9359(2)] was shown to specifically react with mH2A1 in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266241 (knockout cell lysate ab257463) lane below 40kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and mH2A1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab183041 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human H2AFY knockout HEK293T cell lysate (ab257463)Homozygous: 13 bp deletion in exon 2