Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6171(2)(B)] to Histone H2A.Z - ChIP Grade
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, ChIP
Reacts with: Mouse, Rat, Human

Product name

Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade
See all Histone H2A.Z primary antibodies
Description

Rabbit monoclonal [EPR6171(2)(B)] to Histone H2A.Z - ChIP Grade
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP	Human
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Histone H2A.Z aa 1-100. The exact sequence is proprietary.
Positive control
- WB: Neuro-2a, HeLa, HepG2, RAW 264.7, C6 and PC-12 cell lysates. IHC-P: Human colon and lung carcinoma tissues, human breast tissue, mouse and rat cerebrum tissue. ICC/IF: HepG2 and HeLa cells. Flow Cyt: HeLa cells.
General notes
ab150402 is a nuclear marker.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6171(2)(B)
Isotype

IgG
Research areas

Western blot - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

All lanes : Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402) at 1/5000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 4 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 13 kDa
Observed band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling Histone H2A.Z with purified ab150402 at 1/2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
ChIP - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol*. Cells were fixed with formaldehyde for 10 minutes.
The ChIP was performed with 25 µg of chromatin, 2 µg of ab150402 (red), and 20 µl of Protein A/G sepharose beads. 2 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Histone H2A.Z with purified ab150402 at 1/200 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Histone H2A.Z (red) with purified ab150402 at a 1/2500 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling Histone H2A.Z with purified ab150402 at 1:2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling Histone H2A.Z with purified ab150402 at 1/2000 dilution (1.09 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
ChIP - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab150402 (unpurified) (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Histone H2A.Z with ab150402 (unpurified) at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Histone H2A.Z with ab150402 (unpurified) at 1/100 dilution.
Western blot - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402) This image is courtesy of an Abreview submitted by Ragnhild Eskeland

All lanes : Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402) at 1/1000 dilution (unpurified)

Lane 1 : Recombinant Human octamers containing H2A at 1 µg
Lane 2 : Recombinant Human octamers containing H2A at 0.5 µg
Lane 3 : Native recombinant octamers K562 cells at 3 µg
Lane 4 : Native recombinant octamers K562 cells at 1.5 µg
Lane 5 : Recombinant Human octamers containing H2A.Z.2.1 at 0.5 µg
Lane 6 : Recombinant Human octamers containing H2A.Z.1 at 0.5 µg

Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 13 kDa
Observed band size: 15 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Histone H2A.Z with ab150402 (unpurified) at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin embedded human cervical carcinoma tissue using ab150402 (unpurified) showing positive staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin embedded normal human kidney tissue using ab150402 (unpurified) showing positive staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin embedded human prostate hyperplasia tissue using ab150402 showing positive staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using ab150402 (unpurified) showing positive staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Histone H2A.Z antibody [EPR6171(2)(B)] - ChIP Grade (ab150402)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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