Anti-Histone H3.3 antibody (ab62642)
Key features and details
- Rabbit polyclonal to Histone H3.3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3.3 antibody
See all Histone H3.3 primary antibodies -
Description
Rabbit polyclonal to Histone H3.3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H3.3. (synthesized peptide derived from Internal of human Histone H3.3.)
Database link: P84243 -
Positive control
- WB: A431 (Human epithelial carcinoma cell line). ICC: HeLa cells. IHC-P: Human brain tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3.3 antibody (ab62642)Anti-Histone H3.3 antibody (ab62642) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The 19 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Histone H3.3. -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3.3 antibody (ab62642)Immunofluorescence analysis of HeLa cells, using ab62642 at a 1/500 dilution.
Left image untreated.
Right image treated with peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3.3 antibody (ab62642)Immunohistochemistry analysis of paraffin embedded human brain tissue using ab62642 at a 1/50 dilution.
Left image untreated.
Right image treated with peptide.
