Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H1.0 antibody [EPR6536] (ab134914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6536] to Histone H1.0
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H1.0 antibody [EPR6536]
    See all Histone H1.0 primary antibodies

  • Description

    Rabbit monoclonal [EPR6536] to Histone H1.0

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human Histone H1.0 (UniProt: P07305).

  • Positive control

    • WB: A431 and HeLa cell lysates; Human fetal kidney and BxPC3 cell lysates. IHC-P: Human colon tissue. ICC/IF: BxPC3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : H1F0 knockout A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 A431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : BcPC3 cel lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (ab134914)

    Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution.
  • Anti-Histone H1.0 antibody [EPR6536] (ab134914)
    Anti-Histone H1.0 antibody [EPR6536] (ab134914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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