Anti-Histone H1.0 antibody [EPR6536] (ab134914)
![Anti-Histone H1.0 antibody [EPR6536] (ab134914)](https://www.abcam.com/ps/products/134/ab134914/Images/ab134914-387587-anti-histone-h10-antibody-epr6536-western-blot-a431-wt-a431-h1f0-knockout-hela.jpg "Anti-Histone H1.0 antibody [EPR6536] (ab134914)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6536] to Histone H1.0
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Histone H1.0 antibody [EPR6536]
See all Histone H1.0 primary antibodies -
Description
Rabbit monoclonal [EPR6536] to Histone H1.0 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues near the N terminus of Human Histone H1.0 (UniProt: P07305).
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Positive control
- WB: A431 and HeLa cell lysates; Human fetal kidney and BxPC3 cell lysates. IHC-P: Human colon tissue. ICC/IF: BxPC3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6536 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : H1F0 knockout A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab134914 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot. Loss of signal was observed when H1F0 knockout sample was used. Wild-type and H1F0 A431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)](https://www.abcam.com/ps/products/134/ab134914/Images/ab134914-149051-anti-histone-h10-antibody-epr6536-western-blot.jpg)
Western blot - Anti-Histone H1.0 antibody [EPR6536] (ab134914)All lanes : Anti-Histone H1.0 antibody [EPR6536] (ab134914) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : BcPC3 cel lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 21 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (ab134914)](https://www.abcam.com/ps/products/134/ab134914/Images/ab134914-149050-anti-histone-h10-antibody-epr6536-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (ab134914)Immunohistochemical analysis of Histone H1.0 in paraffin embedded Human colon tissue, using ab134914 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (ab134914)](https://www.abcam.com/ps/products/134/ab134914/Images/ab134914-149049-anti-histone-h10-antibody-epr6536-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.0 antibody [EPR6536] (ab134914)Immunofluorescent analysis of Histone H1.0 in BxPC3 cells, using ab134914 at a 1/100 dilution. -
![Anti-Histone H1.0 antibody [EPR6536] (ab134914)](https://www.abcam.com/ps/products/134/ab134914/Images/ab134914-5-benefits-of-recombinant-antibodies.png)
Anti-Histone H1.0 antibody [EPR6536] (ab134914)
