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Cancer Tumor immunology Cytokines Interleukins

Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI2F4] to IL36 gamma/IL-1F9
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-IL36 gamma/IL-1F9 antibody [OTI2F4]
    See all IL36 gamma/IL-1F9 primary antibodies
  • Description

    Mouse monoclonal [OTI2F4] to IL36 gamma/IL-1F9
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human IL36 gamma/IL-1F9 aa 1-169.
    Sequence:

    MRGTPGDADGGGRAVYQSMCKPITGTINDLNQQVWTLQGQNLVAVPRSDS VTPVTVAVITCKYPEALEQGRGDPIYLGIQNPEMCLYCEKVGEQPTLQLK EQKIMDLYGQPEPVKPFLFYRAKTGRTSTLESVAFPDWFIASSKRDQPII LTSELGKSYNTAFELNIND


    Database link: NP_062564
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HEK293T cells transfected with pCMV6-ENTRY IL-36 gamma/IL-1F9 cDNA; Human colon adenocarcinoma and pancreas tissues; HeLa cells
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 2F4 to OTI2F4, both clone numbers name the same clone.

    This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Previously labelled as IL36 gamma. 

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab156783 is purified from TCS by affinity chromatography
  • Clonality

    Monoclonal
  • Clone number

    OTI2F4
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Images

  • Western blot - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
    Western blot - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
    All lanes : Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control

    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY IL36 gamma/IL-1F9 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 19 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC213525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution

  • Immunocytochemistry/ Immunofluorescence - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)
    Immunocytochemistry/ Immunofluorescence - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783)

    Immunofluorescent analysis of HeLa cells labeling IL36 gamma/IL-1F9 using ab156783 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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