Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-16 antibody [EPR19988] (ab207181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19988] to IL-16
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-16 antibody [EPR19988]
    See all IL-16 primary antibodies

  • Description

    Rabbit monoclonal [EPR19988] to IL-16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: H9 and Jurkat whole cell lysates; Human thymus, spleen and tonsil lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: H9 cells. Flow Cyt: H9 cells. IP: H9 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19988

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IL-16 antibody [EPR19988] (ab207181)
    Western blot - Anti-IL-16 antibody [EPR19988] (ab207181)
    All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution

    Lane 1 : H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75-40,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-16 antibody [EPR19988] (ab207181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-16 antibody [EPR19988] (ab207181)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human tonsil is observed [PMID: 10946273]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-16 antibody [EPR19988] (ab207181)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-16 antibody [EPR19988] (ab207181)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16 with ab207181 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on H9 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-IL-16 antibody [EPR19988] (ab207181)
    Western blot - Anti-IL-16 antibody [EPR19988] (ab207181)
    All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution

    Lane 1 : Human thymus lysate at 10 µg
    Lane 2 : Human spleen lysate at 10 µg
    Lane 3 : Human tonsil lysate at 20 µg

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa
    Observed band size: 75-40,80 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds, Lane 2: 15 seconds, Lane 3: 3 minutes.

    The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-16 antibody [EPR19988] (ab207181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-16 antibody [EPR19988] (ab207181)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on stromal cells of human colon is observed [PMID: 11709514]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-IL-16 antibody [EPR19988] (ab207181)
    Flow Cytometry - Anti-IL-16 antibody [EPR19988] (ab207181)

    Flow cytometric analysis of 4% paraformaldehyde-fixed H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16 with ab207181 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-IL-16 antibody [EPR19988] (ab207181)
    Immunoprecipitation - Anti-IL-16 antibody [EPR19988] (ab207181)

    IL-16 was immunoprecipitated from 0.35 mg of H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate with ab207181 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207181 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: H9 whole cell lysate, 10 μg (Input).

    Lane 2: ab207181 IP in H9 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207181 in H9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Note: The band at around 50kDa is a cleaved form of IL-16.

     

  • Anti-IL-16 antibody [EPR19988] (ab207181)
    Anti-IL-16 antibody [EPR19988] (ab207181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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