Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL2 antibody [EPR23331-159] (ab275879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23331-159] to CXCL2
  • Suitable for: IP, WB
  • Reacts with: Rat

Overview

  • Product name

    Anti-CXCL2 antibody [EPR23331-159]
    See all CXCL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23331-159] to CXCL2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Rat
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat lung organ (treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant ;Recombinant Rat CXCL2 protein. IP: Rat lung (organ treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23331-159

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    All lanes : Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution

    Lane 1 : Untreated rat lung organ culture, supernatant
    Lane 2 : Rat lung organ (treated with 10/ml Concanavalin A for 3 days) culture, supernatant

    Lysates/proteins at 10 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 11 kDa
    Observed band size: 10 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15  seconds.

     

  • Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] (ab275879)

    CXCL2 was immunoprecipitated from 0.35 mg Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant with ab275879 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275879 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant 10 μl

    Lane 2: ab275879 IP in Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275879 in rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    Western blot - Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution + Recombinant Rat CXCL2 protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 10 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6  seconds.

     

  • Anti-CXCL2 antibody [EPR23331-159] (ab275879)
    Anti-CXCL2 antibody [EPR23331-159] (ab275879)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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