Human CXCL5 Matched Antibody Pair Kit (ab215074)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Human CXCL5 Matched Antibody Pair Kit
See all CXCL5 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.48 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human CXCL5 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CXCL5.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212163. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human CXCL5 Capture Antibody 2 x 50µg 1 x 50µg Human CXCL5 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human CXCL5 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AMCFII
- C-X-C motif chemokine 5
- C-X-C motif chemokine ligand 5
see all -
Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
Images
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Human CXCL5 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human CXCL5 Matched Antibody Pair Kit (ab215074)To learn more about the advantages of recombinant antibodies see here.
