Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
![Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107749-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg "Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3132]
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3132] to CD90 / Thy1 -
Host species
Rabbit -
Specificity
This antibody reacts with mouse samples in WB, but does not work on mouse samples in other applications. Rat cross-reactivity has been tested by western blot only.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human CD90/ Thy1 aa 50-150 (N terminal).
Database link: P04216 -
Positive control
- Fetal brain, fetal kidney lysates, Human breast carcinoma, Human glioma and Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.57 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3132 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107750-anti-cd90-thy1-antibody-epr3132-western-blot.jpg)
Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107748-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in Normal human tonsil vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107747-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Skeletal muscle vessels tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107746-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Cervical carcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107745-anti-cd90-thy1-antibody-epr3132-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)ab92574 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-107744-anti-cd90-thy1-antibody-epr3132.jpg)
OI-RD Scanning - Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) -
![Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)](https://www.abcam.com/ps/products/92/ab92574/Images/ab92574-8-benefits-of-recombinant-antibodies.png)
Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
