HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
![HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)](https://www.abcam.com/ps/products/199/ab199219/Images/ab199219-245064-WBab1992191.jpg "HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)")
Key features and details
- HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
See all CD90 / Thy1 primary antibodies -
Description
HRP Mouse monoclonal [MRC OX-7] to CD90 / Thy1 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Full length protein corresponding to Rat CD90/ Thy1.
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Positive control
- WB: Rat brain tissue lysate and PC12 whole cell lysate.
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General notes
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-7 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)All lanes : HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219) at 1/5000 dilution
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199219 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
