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Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase
  • Suitable for: Flow Cyt, WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325]
    See all Nucleoside triphosphate phosphohydrolase primary antibodies

  • Description

    Rabbit monoclonal [EPR14325] to Nucleoside triphosphate phosphohydrolase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, U87-MG and Jurkat cell lysates. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14325

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : NTPCR knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab182154 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab182154 Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] was shown to specifically react with Nucleoside triphosphate phosphohydrolase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266397 (knockout cell lysate ab258083) was used. Wild-type and Nucleoside triphosphate phosphohydrolase knockout samples were subjected to SDS-PAGE. ab182154 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    Flow Cytometry - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling Nucleoside triphosphate phosphohydrolase with ab182154 at 1/230 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    Western blot - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    All lanes : Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154) at 1/2000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa

  • Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    Immunoprecipitation - Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

    Western blot analysis of immunoprecipitation pellet from U87-MG cell lysate immunoprecipitated using ab182154 at 1/20 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)
    Anti-Nucleoside triphosphate phosphohydrolase antibody [EPR14325] (ab182154)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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