Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Beclin 1 antibody [EPR19662] (ab207612)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19662] to Beclin 1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Beclin 1 antibody [EPR19662]
See all Beclin 1 primary antibodies
Description

Rabbit monoclonal [EPR19662] to Beclin 1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, HEK-293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19662
Isotype

IgG
Research areas

Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 6 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 30 seconds; Lane 5: 15 seconds; Lane 6: 15 seconds.
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

Lanes 1-4 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution
Lanes 5-6 : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/5000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 5 seconds; Lane 5: 10 seconds; Lane 6: 30 seconds.
Western blot - Anti-Beclin 1 antibody [EPR19662] (ab207612)

All lanes : Anti-Beclin 1 antibody [EPR19662] (ab207612) at 1/2000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/6: 5 seconds; Lane 2/3: 15 seconds; Lane 4: 3 seconds; Lane 5: 10 seconds.
Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] (ab207612)

Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10µg (Input).

Lane 2: ab207612 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Beclin 1 antibody [EPR19662] (ab207612)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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