Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Beclin 1 antibody [EPR20473] (ab210498)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20473] to Beclin 1
Suitable for: IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Beclin 1 antibody [EPR20473]
See all Beclin 1 primary antibodies
Description

Rabbit monoclonal [EPR20473] to Beclin 1
Host species

Rabbit
Specificity

We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HepG2, HEK-293, T-47D, Raji, RAW 264.7, NIH/3T3, A20 and C2C12 whole cell lysates; Human fetal brain and colon lysates; Mouse brain and spleen lysates; Rat brain, kidney and spleen lysates. IHC-P: Human kidney and breast cancer tissues. IP: HepG2 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20473
Isotype

IgG
Research areas

Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)

All lanes : Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 5 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] (ab210498)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Granularly cytoplasmic staining on human kidney is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] (ab210498)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Granularly cytoplasmic staining on tumor cells of human breast cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)

All lanes : Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human colon lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)

All lanes : Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg
Lane 3 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg
Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Beclin 1 antibody [EPR20473] (ab210498)

All lanes : Anti-Beclin 1 antibody [EPR20473] (ab210498) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat kidney lysate
Lane 5 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 52 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] (ab210498)

Beclin 1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab210498 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab210498 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HepG2 whole cell lysate, 10 µg (Input).

Lane 2: ab210498 IP in HepG2 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210498 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Beclin 1 antibody [EPR20473] (ab210498)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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