Anti-Beclin 1 (phospho S234) antibody (ab183335)
Key features and details
- Rabbit polyclonal to Beclin 1 (phospho S234)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 (phospho S234) antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 (phospho S234) -
Host species
Rabbit -
Specificity
Immunolabeling of the Beclin 1 band is decreased in the Beclin 1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Beclin 1 (phospho S234). The phosphopeptide corresponds to amino acid residues surrounding phospho-serine 234.
Database link: Q14457 -
Positive control
- HeLa and 293T expressing recombinant Beclin 1 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183335 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Beclin 1 (phospho S234) antibody (ab183335)All lanes : Anti-Beclin 1 (phospho S234) antibody (ab183335) at 1/250 dilution
Lane 1 : Lysate from 293T cells expressing wild type flag-Beclin 1
Lane 2 : Lysate from 293T cells expressing mutant flag-Beclin 1 (S234A, S295A).
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
