Anti-Beclin 1 (phospho S295) antibody (ab183313)
Key features and details
- Rabbit polyclonal to Beclin 1 (phospho S295)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Beclin 1 (phospho S295) antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 (phospho S295) -
Host species
Rabbit -
Specificity
ab183313 only detects Beclin 1 when phosphorylated at serine 295. Immunolabeling of the Beclin 1 band is eliminated in the Beclin 1 alanine substitution mutant. Additional bands may be observed in non-immunoprecipitated lysates. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human Beclin 1 (phospho S295).
Database link: Q14457 -
Positive control
- DDDDK Beclin 1 WT immunoprecipitates expressed in 293T cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol, 0.01% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183313 was prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Beclin 1 (phospho S295) antibody (ab183313)All lanes : Anti-Beclin 1 (phospho S295) antibody (ab183313) at 1/250 dilution
Lane 1 : DDDDK Beclin 1 WT immunoprecipitates expressed in 293T cells
Lane 2 : DDDDK Beclin 1 alanine-substitution mutant (S295A) immunoprecipitates expressed in 293T cells
Predicted band size: 52 kDa
