Anti-SESN2/Sestrin-2 antibody (ab264413)
Key features and details
- Rabbit polyclonal to SESN2/Sestrin-2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SESN2/Sestrin-2 antibody
See all SESN2/Sestrin-2 primary antibodies -
Description
Rabbit polyclonal to SESN2/Sestrin-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SESN2/Sestrin-2 aa 275-325. The exact sequence is proprietary.
Database link: P58004 -
Positive control
- WB: HEK-293T, RKO and K562 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SESN2/Sestrin-2 antibody (ab264413)All lanes : Anti-SESN2/Sestrin-2 antibody (ab264413) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-SESN2/Sestrin-2 antibody (ab264413)SESN2/Sestrin-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264413 at 100 µl per reaction. Western blot was performed from the immunoprecipitate using ab264413 at 1/400 dilution.
Lane 1: ab264413 IP in HEK-293T whole cell lysate.
Lane 2: Control IgGIP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
Lysates prepared using NETN lysis buffer.
