Anti-TTF1 antibody [EPR8190-6] (ab133638)
![Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147553-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg "Anti-TTF1 antibody [EPR8190-6] (ab133638)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8190-6] to TTF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TTF1 antibody [EPR8190-6]
See all TTF1 primary antibodies -
Description
Rabbit monoclonal [EPR8190-6] to TTF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)
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Positive control
- HeLa, Human lung adenocarcinoma, fetal lung, mouse lung and rat lung lysates; Human papillary thyroid carcinoma and lung carcinoma tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8190-6 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)
ab133638 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147555-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147557-anti-ttf1-antibody-epr8190-6-western-blot.jpg)
Western blot - Anti-TTF1 antibody [EPR8190-6] (ab133638)All lanes : Anti-TTF1 antibody [EPR8190-6] (ab133638) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : Human lung adenocarcinoma lysate
Lane 3 : Fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
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![Western blot - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147556-anti-ttf1-antibody-epr8190-6-western-blot.jpg)
Western blot - Anti-TTF1 antibody [EPR8190-6] (ab133638)All lanes : Anti-TTF1 antibody [EPR8190-6] (ab133638) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147554-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with ab133638 at 1/500 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147552-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)ab133638 showing positive staining in human Thyroid gland tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147551-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)ab133638 showing negative staining in Normal human iver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147550-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)ab133638 showing positive staining in human Small cell lung carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147549-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)ab133638 showing negative staining in human Lung squamous carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-147548-anti-ttf1-antibody-epr8190-6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190-6] (ab133638)ab133638 showing negative staining in human Normal lung tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TTF1 antibody [EPR8190-6] (ab133638)](https://www.abcam.com/ps/products/133/ab133638/Images/ab133638-11-benefits-of-recombinant-antibodies.png)
Anti-TTF1 antibody [EPR8190-6] (ab133638)
