Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1584Y] to TTF1
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TTF1 antibody [EP1584Y]
See all TTF1 primary antibodies
Description

Rabbit monoclonal [EP1584Y] to TTF1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human TTF1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P43699
(Peptide available as ab187893)
Positive control
- IHC-P: Human lung carcinoma and thyroid carcinoma tissue; Rat lung tissue; Mouse lung tissue. WB: HeLa and HEK-293 cell lysate; Rat and mouse lung lysate. Flow Cyt: HeLa and A549 cells. IHC-Fr: Murine fetal lung tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1584Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)

Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/10000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 38-42 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)

Anti-TTF1 antibody [EP1584Y] (ab76013) (purified) + Rat lung lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013)

Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (purified) + Mouse lung lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Western blot - Anti-TTF1 antibody [EP1584Y] (ab76013) This image is courtesy of an anonymous Abreview

Anti-TTF1 antibody [EP1584Y] (ab76013) at 1/2000 dilution (unpurified) + HEK293 whole cell lysate at 30 µg

Secondary
HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 38-42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Exposure time: 2 minutes

Blocked with 5% milk
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EP1584Y] (ab76013)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TTF1 with purified ab76013 at 1/250 dilution (0.6 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
Anti-TTF1 antibody [EP1584Y] (ab76013)

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