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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)

Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR101B] to NOTCH4 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free
    See all NOTCH4 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR101B] to NOTCH4 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Data from our lab shows that this antibody does not work in WB on Human samples but does react with endogenous mouse protein. Other species have not been tested.

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249346 is the carrier-free version of ab166605 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249346 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between the National Cancer Institutes Center for Cancer Research and the lab of Robert Callahan. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR101B
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

Images

  • Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
    Western blot - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
    Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution + Mouse lung lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 210 kDa



    This data was developed using ab166605, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
    Flow Cytometry - Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
    This data was developed using ab166605, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized Jurkat cells with ab166605 antibody at a dilution of 1/100 (red) compared to a rabbit IgG negative (green).
  • Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)
    Anti-NOTCH4 antibody [EPNCIR101B] - BSA and Azide free (ab249346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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