Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2
- Suitable for: WB, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-AKR1C1/AKR1C2 antibody [EPR11542]
See all AKR1C1/AKR1C2 primary antibodies -
Description
Rabbit monoclonal [EPR11542] to AKR1C1/AKR1C2 -
Host species
Rabbit -
Specificity
AKR1C1 and AKR1C2 (Swiss Prot: Q04828/P52895) sequences are 98% identical -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AKR1C1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q04828 -
Positive control
- HepG2, U87-MG, Human fetal liver and HeLa lysates; U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11542 -
Isotype
IgG -
Research areas
Images
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Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab179448 at 1/10 dilution.
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Flow cytometric analysis of permeabilized U87-MG cells labeling AKR1C1/AKR1C2 with ab179448 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
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All lanes : Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : U87-MG lysate
Lane 3 : Human fetal liver lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
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