HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)
![HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)](https://www.abcam.com/ps/products/198/ab198411/Images/ab198411-238822-IHCap22494591.jpg "HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)")
Key features and details
- HRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3]
See all AKR1C1/AKR1C2 primary antibodies -
Description
HRP Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Full length native protein (purified) corresponding to Human AKR1C1/AKR1C2.
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Positive control
- IHC-P: Normal human liver tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
4B6AF3 -
Isotype
IgG2b -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab198411)
IHC image of AKR1C1/AKR1C2 staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198411, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
