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Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free
  • Suitable for: IP, IHC-P, ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-Aurora B antibody [EP1009Y] - BSA and Azide free
    See all Aurora B primary antibodies

  • Description

    Rabbit monoclonal [EP1009Y] to Aurora B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa whole cell lysate; Flow Cyt (intra): HeLa; WB: HeLa cell lysate; IHC: human endometrium carcinoma; ICC/IF: HeLa cells.

  • General notes

    ab239837 is the carrier-free version of ab45145.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Flow Cytometry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    Flow Cytometry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    This data was developed using ab45145, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:300 dilution (0.1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left). Unlabeled control - /.
  • Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    Immunocytochemistry - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

    This data was developed using ab45145, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Aurora B with Purified ab45145 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    Immunoprecipitation - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

    This data was developed using ab45145, the same antibody clone in a different buffer formulation.
    Purified ab45145 at 1/20 dilution (1µg) immunoprecipitating Aurora B in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab45145 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45145 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 39 kDa

  • OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    OI-RD Scanning - Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45145).

  • Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)
    Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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