Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Kinases Aurora

Anti-Aurora B antibody (ab70238)

Anti-Aurora B antibody (ab70238)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Aurora B
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-Bora antibody [EPR4456] - BSA and Azide free (ab247647)
R428 (BGB324), AXL inhibitor (ab141364)
Product image
APC Anti-Aurora B + Aurora A (phospho T288) + IPL-1/STK13/Aurora C antibody [AuroraABC-CC12] (ab278629)
Product image
Anti-Aurora B antibody (ab2254)

Overview

  • Product name

    Anti-Aurora B antibody
    See all Aurora B primary antibodies
  • Description

    Rabbit polyclonal to Aurora B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).

  • Positive control

    • IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Aurora
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Phosphorylation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Aurora
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Aurora

Images

  • Immunocytochemistry - Anti-Aurora B antibody (ab70238)
    Immunocytochemistry - Anti-Aurora B antibody (ab70238)
    Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
  • Immunoprecipitation - Anti-Aurora B antibody (ab70238)
    Immunoprecipitation - Anti-Aurora B antibody (ab70238)
    Detection of Aurora B by immunoprecipitation of whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Lane 1: ab70238 at 0.1 µg/mL. Lane 2: IgG Control. Detection: Chemiluminescence with an exposure time of 3 minutes.
  • Western blot - Anti-Aurora B antibody (ab70238)
    Western blot - Anti-Aurora B antibody (ab70238)
    All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilution

    Lanes 1 & 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)

    Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)

    Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa
    Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.



    Detection time: 10 secs (lanes 1,2) or 30 secs (lanes 3,4)
  • Western blot - Anti-Aurora B antibody (ab70238)
    Western blot - Anti-Aurora B antibody (ab70238)
    All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate from an asynchronous culture
    Lane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hours
    Lane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hours
    Lane 4 : HeLa whole cell lysate
    treated with cycloheximide for 36 hours
    Lane 5 : HeLa whole cell lysate treated with nocodazole for 20 hours

    Lysates/proteins at 100 µg per lane.

    Predicted band size: 39 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Aurora B antibody (ab70238)

  •  
  • Product image

    Anti-Aurora B antibody [EP1009Y] (ab45145)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-Aurora B (phospho S227) antibody [EPR20389] (ab210706)

    Applications: Dot, ICC/IF, IP, WB

  •  
  • Product image

    Anti-Aurora B (phospho T232) antibody (ab115793)

    Applications: IHC-P

  •  
  • Product image

    Anti-Aurora B antibody [EP1009Y] - BSA and Azide free (ab239837)

    Applications: Flow Cyt, ICC, IHC-P, IP, WB

  •  
  • Product image

    Anti-Aurora B antibody [mAbcam 3609] (ab3609)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-Aurora B antibody (ab2254)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-Aurora B antibody [EP1009Y] (ab197614)

    Applications: ICC/IF

Clear all

Recently viewed products

  •  
  • Product image

    Anti-CAPZB antibody [EPR10236] (ab175212)

  •  
  • Product image

    Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

  •  
  • Product image

    Anti-Cyclin H/p34 antibody [EPR3929] (ab92376)

  •  
  • Product image

    Anti-LAT antibody [Y109] (ab32070)

  •  
  • Product image

    Anti-SOX18 antibody [EPR2837(2)] (ab109194)

  •  
  • Product image

    Anti-CCL21 antibody [EPR6218] (ab129014)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.