Anti-Aurora B antibody (ab70238)
Key features and details
- Rabbit polyclonal to Aurora B
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Aurora B antibody
See all Aurora B primary antibodies -
Description
Rabbit polyclonal to Aurora B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIP HumanWB Human -
Immunogen
Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).
-
Positive control
- IP: Lysate from nocodazole treated HeLa cells; WB: Lysates from nocodazole treated HeLa cells; ICC: HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
-
Detection of Aurora B by immunoprecipitation of whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Lane 1: ab70238 at 0.1 µg/mL. Lane 2: IgG Control. Detection: Chemiluminescence with an exposure time of 3 minutes.
-
All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilution
Lanes 1 & 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Detection time: 10 secs (lanes 1,2) or 30 secs (lanes 3,4) -
All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate from an asynchronous culture
Lane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hours
Lane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hours
Lane 4 : HeLa whole cell lysate
treated with cycloheximide for 36 hours
Lane 5 : HeLa whole cell lysate treated with nocodazole for 20 hours
Lysates/proteins at 100 µg per lane.
Predicted band size: 39 kDa