Anti-Aurora B antibody [mAbcam 3609] (ab3609)
Key features and details
- Mouse monoclonal [mAbcam 3609] to Aurora B
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Aurora B antibody [mAbcam 3609]
See all Aurora B primary antibodies -
Description
Mouse monoclonal [mAbcam 3609] to Aurora B -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Aurora B aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab13569) -
Positive control
- Hela whole cell lysate
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
mAbcam 3609 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1 µg/ml
Lane 1 : Hek293 Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate
Lane 3 : HeLa Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate- Nocodozole Stimulated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Absorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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SKN-SH cells were fixed in 4% paraformaldehyde for 10 mins, permeabilized in PBS-0.5% Triton X-100 for 5 mins and incubated for 30 minutes with ab3609 (1/50 dilution). The slides were blocked with 4mg/ml BSA. The slides were rinsed once in PBS-Triton (0.1%), twice in PBS then incubated with the secondary antibody for 30 mins. The DNA is stained with DAPI (blue). Staining with ab3609 can be seen in green.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [mAbcam 3609] (ab3609) Image courtesy of Human Protein AtlasImage courtesy of Human Protein Atlas
Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody [mAbcam 3609] (ab3609) Image courtesy of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canadaab3609 (1/200) staining Aurora B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus/ chromosomes (red). For further experimental details please refer to Abreview.