Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-B7-H6 antibody [EPR23470-210] (ab253180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23470-210] to B7-H6
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-B7-H6 antibody [EPR23470-210]
    See all B7-H6 primary antibodies

  • Description

    Rabbit monoclonal [EPR23470-210] to B7-H6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and K562 whole cell lysates. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23470-210

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-B7-H6 antibody [EPR23470-210] (ab253180)
    Western blot - Anti-B7-H6 antibody [EPR23470-210] (ab253180)
    All lanes : Anti-B7-H6 antibody [EPR23470-210] (ab253180) at 1/1000 dilution

    Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 whole cell lysate treated with PNGase F 20
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 50,80 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Negative control: MCF7.

    The expression profile is consistent with the literature (PMID: 22851709).
    B7-H6 is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID: 24780758).

     

  • Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] (ab253180)
    Immunoprecipitation - Anti-B7-H6 antibody [EPR23470-210] (ab253180)

    B7-H6 was immunoprecipitated from 0.35 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate  with ab253180 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253180 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate 10 μg

    Lane 2: ab253180 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253180 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds.

    The lower bands are likely to be the deglycosylated form of B7-H6.

  • Anti-B7-H6 antibody [EPR23470-210] (ab253180)
    Anti-B7-H6 antibody [EPR23470-210] (ab253180)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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