Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AP1] to APLP1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-APLP1 antibody [AP1] - BSA and Azide free
    See all APLP1 primary antibodies

  • Description

    Mouse monoclonal [AP1] to APLP1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain cortex tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue; Human cerebrum Alzheimer's disease tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273469 is the carrier-free version of ab273448. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Western blot - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Anti-APLP1 antibody [AP1] (ab273448) at 1/1000 dilution + Mouse brain cortex tissue lysate at 20 µg

    Secondary
    Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 72 kDa


    Exposure time: 37 seconds


    This data was developed using ab273448, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

    This data was developed using ab273448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

    This data was developed using ab273448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum with Alzheimer's disease tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on human cerebrum with Alzheimer's disease is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

    This data was developed using ab273448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on mouse cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

    This data was developed using ab273448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling APLP1 with ab273448 at 1/500 dilution followed by a ready to use secondary. Positive staining on rat cerebrum is observed. The section was incubated with ab273448 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use secondary.
    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)
    Anti-APLP1 antibody [AP1] - BSA and Azide free (ab273469)

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