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Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)

Key features and details

  • Mouse monoclonal [Rab7-117] to RAB7 - Late Endosome Marker
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker
    See all RAB7 primary antibodies

  • Description

    Mouse monoclonal [Rab7-117] to RAB7 - Late Endosome Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide:

    EQAFQTIARNALKQE

    , corresponding to amino acids 163-177 of Human RAB7
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLA, HepG2, NRK , NIH3/T3 and MDCK cell lysates. ICC: HeLa cells.

Properties

Images

  • Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    All lanes : Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : NRK (rat cell kidney line) cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
    Lane 5 : MDCK (dog kidney cell line) cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-peroxidase

    Predicted band size: 23 kDa

  • Immunocytochemistry - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Immunocytochemistry - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling RAB7 with ab50533 at 10 μg/mL. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% saponin. Goat Anti-Mouse IgG, Atto-488 conjugate was used as the secondary antibody.
     

     

  • Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) This image is courtesy of an Abreview submitted by Armen Petrosyan
    Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution + HepG2 whole cell lysate at 1/2000 dilution

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 30 seconds


    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • Immunocytochemistry - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Immunocytochemistry - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) This image is courtesy of an Abreview submitted by Armen Petrosyan

    ab50533 staining RAB7 in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in PBST + 1% donkey serum) for 3 hours at 22°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) This image is courtesy of an Abreview submitted by Armen Petrosyan
    Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/2000 dilution + Rat hepatocytes whole cell lysate at 20 µg

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 30 seconds


    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hour at 4°C in PBS + 1% BSA.

    See Abreview

  • Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) This image is a courtesy of Anonymous Abreview
    Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/1000 dilution + Lysate prepared from mouse neuroblastoma cells at 10 µg

    Secondary
    HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 20 seconds

    See Abreview

  • Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533)
    Western blot - Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) This image is courtesy of an anonymous Abreview
    Anti-RAB7 antibody [Rab7-117] - Late Endosome Marker (ab50533) at 1/500 dilution (for 3 hours at 20°C) + Human brain whole tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 1 hour at 20°C

    See Abreview

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