Anti-RAB7 antibody (ab229647)
Key features and details
- Rabbit polyclonal to RAB7
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB7 antibody
See all RAB7 primary antibodies -
Description
Rabbit polyclonal to RAB7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Dog, Xenopus laevis
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Immunogen
Synthetic peptide within Human RAB7 (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P51149 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Mouse liver and rat intestine tissues. ICC/IF: A431 cells and DIV9 rat E18 primary cortical neurons.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB7 antibody (ab229647)All lanes : Anti-RAB7 antibody (ab229647) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody (ab229647)4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Rab7 (green) using ab229647 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody (ab229647)Paraffin-embedded mouse liver tissue stained for Rab7 using ab229647 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody (ab229647)Paraffin-embedded rat intestine tissue stained for Rab7 using ab229647 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody (ab229647)4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Rab7 (green) using ab229647 at 1/500 dilution in ICC/IF.
Red: beta Tubulin 3 stained by beta Tubulin 3 antibody at 1/500 dilution.
Blue: DAPI.
