Anti-ENPP2/ATX antibody [1F8] (ab77104)
![Anti-ENPP2/ATX antibody [1F8] (ab77104)](https://www.abcam.com/ps/products/77/ab77104/Images/ab77104-92784-anti-enpp2-antibody-5h3-immunohistochemistry.jpg "Anti-ENPP2/ATX antibody [1F8] (ab77104)")
Key features and details
- Mouse monoclonal [1F8] to ENPP2/ATX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-ENPP2/ATX antibody [1F8]
See all ENPP2/ATX primary antibodies -
Description
Mouse monoclonal [1F8] to ENPP2/ATX -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Recombinant full length protein corresponding to Human ENPP2/ATX.
-
Positive control
- WB: HEK293 whole cell lysate and in the following human tissue lysates: kidney; placenta; ovary; small intestine. IHC: human tonsil paraffin sections. ICC/IF: HeLa cells.
-
General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
This product was previously labelled as ENPP2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
1F8 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENPP2/ATX antibody [1F8] (ab77104)
IHC image of ENPP2/ATX staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77104, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
-
![Western blot - Anti-ENPP2/ATX antibody [1F8] (ab77104)](https://www.abcam.com/ps/products/77/ab77104/Images/ENPP2-Primary-antibodies-ab77104-15.jpg)
Western blot - Anti-ENPP2/ATX antibody [1F8] (ab77104)All lanes : Anti-ENPP2/ATX antibody [1F8] (ab77104) at 10 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa, 250 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
-
![Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody [1F8] (ab77104)](https://www.abcam.com/ps/products/77/ab77104/Images/ENPP2-Primary-antibodies-ab77104-18.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENPP2/ATX antibody [1F8] (ab77104)ICC/IF image of ab77104 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77104, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Western blot - Anti-ENPP2/ATX antibody [1F8] (ab77104)](https://www.abcam.com/ps/products/77/ab77104/Images/ENPP2-Primary-antibodies-ab77104-16.jpg)
Western blot - Anti-ENPP2/ATX antibody [1F8] (ab77104)All lanes : Anti-ENPP2/ATX antibody [1F8] (ab77104) at 10 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 40 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesENPP2/ATX contains three potential glycosylation sites (SwissProt), which might explain its migration at a higher molecular weight than predicted.
