Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-EIF2S2/EIF2B antibody [EPR15833(B)]
    See all EIF2S2/EIF2B primary antibodies

  • Description

    Rabbit monoclonal [EPR15833(B)] to EIF2S2/EIF2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa, 293T and Jurkat cell lysates. HeLa and 293 cells.

  • General notes

     

     

     This product was previously labelled as EIF2S2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    All lanes : Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

    Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2/EIF2B using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution, counterstained with Dapi.

  • Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

    Western blot analysis of EIF2S2/EIF2B in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.

  • Flow Cytometry - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    Flow Cytometry - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

    Flow cytometric analysis of 2% paraformaldehyde fixed 293 cells labeling EIF2S2/EIF2B using ab184549 at a 1/160 dilution (red) or a Rabbit monoclonal IgG control (green).

    Secondary antibody: Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution

  • Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)
    Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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