Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7588(B)] to RAB7 - Late Endosome Marker
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker
    See all RAB7 primary antibodies

  • Description

    Rabbit monoclonal [EPR7588(B)] to RAB7 - Late Endosome Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RAB7 aa 50-150. The exact sequence is proprietary.
    Database link: P51149

  • Positive control

    • WB: A375, A431, HACAT, B16F0 and C6 cell lysates and human fetal brain tissue lysate. IHC-P: Human colon adenocarcinoma tissue. ICC/IF: HT-29 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: RAB7 knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Human fetal brain tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126712 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab126712 was shown to recognize RAB7 when RAB7 knockout samples were used, along with additional cross-reactive bands. Wild-type and RAB7 knockout samples were subjected to SDS-PAGE. ab126712 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Flow Cytometry - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

    ab126712 staining RAB7 in the human cell line A431 (human epidermoid carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

     Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma epithelial cell) labeling RAB7 with ab126712 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

    Confocal image showing cytoplasmic staining in HT-29 cells.

     

  • Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    All lanes : Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712) at 1/1000 dilution

    Lane 1 : A375 whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    All lanes : Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712) at 1/1000 dilution

    Lane 1 : C6 (rat glioma) whole cell lysate
    Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Western blot - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    All lanes : Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712) at 1/1000 dilution (unpurified)

    Lane 1 : A375 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HACAT cell lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : B16F0 cell lysate
    Lane 6 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

    Immunohistochemical staining of paraffin-embedded human bladder carcinoma sections labelling RAB7 with purified ab126712 at dilution of 1/70. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

    Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling Rab7A with unpurified ab126712 at dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

    Immunocytochemistry/immunofluorescence staining of 4% paraformaldehyde fixed; 0.1% triton X 100 permeabilized HT-29 (human colorectal adenocarcinoma) cells with purified ab126712 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

  • OI-RD Scanning - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    OI-RD Scanning - Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)
    Anti-RAB7 antibody [EPR7588(B)] - Late Endosome Marker (ab126712)

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