Anti-Amyloid Precursor Protein antibody [1B5] (ab273450)
![Anti-Amyloid Precursor Protein antibody [1B5] (ab273450)](https://www.abcam.com/ps/products/273/ab273450/Images/ab273450-1-anti-amyloid-protofibrils-antibody-1b5-immunohistochemistry-human-alzheimers-disease-cerebrum.jpg "Anti-Amyloid Precursor Protein antibody [1B5] (ab273450)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1B5] to Amyloid Precursor Protein
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Amyloid Precursor Protein antibody [1B5]
See all Amyloid Precursor Protein primary antibodies -
Description
Mouse monoclonal [1B5] to Amyloid Precursor Protein -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human Alzheimer's disease cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1B5 -
Isotype
IgG2b -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Not generated yet. (ab273450)
Immunohistochemical analysis of paraffin-embedded human Alzheimer's disease cerebrum tissue labeling Amyloid protofibrils with ab273450 at 1/500 dilution followed by a ready to use secondary. Positive staining on human Alzheimer's disease cerebrum tissue is observed. The section was incubated with ab273450 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab190482 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Not generated yet. (ab273450)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Amyloid protofibrils with ab273450 at 1/500 dilution followed by a ready to use secondary. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Negative control: Human cerebrum.
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![Anti-Amyloid Precursor Protein antibody [1B5] (ab273450)](https://www.abcam.com/ps/products/273/ab273450/Images/ab273450-3-benefits-of-recombinant-antibodies.png)
Anti-Amyloid Precursor Protein antibody [1B5] (ab273450)
