Anti-RAGE antibody [EPR12205] (ab181369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12205] to RAGE
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RAGE antibody [EPR12205]
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR12205] to RAGE -
Host species
Rabbit -
Specificity
Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal lung and mouse lung lysates.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12205 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAGE antibody [EPR12205] (ab181369) at 1/1000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lane 6 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and dilution buffer: 5% NFDM/TBST.
The expression profile and molecular mass observed is consistent with what has been described in the literature (PMID: 16315007; 18355449; 18245812).
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All lanes : Anti-RAGE antibody [EPR12205] (ab181369) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
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