Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7074(N)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR7074(N)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WB, IP, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The immunogen used for this product is within Human Amyloid Precursor Protein aa 750 to the C-terminus and therefore may detect gamma secretase fragments 50, 57 and 59 in addition to fragments C31, C80, C83 and C99. Cross-reactivity with these fragments has not been confirmed experimentally.

    Ab251447 is the carrier-free version of ab206297. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251447 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human hippocampus treated with Lambda Phosphatase lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100-120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab206297, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    This data was developed using ab206297, the same antibody clone in a different buffer formulation.Dot blot analysis of Amyloid Precursor Protein (phospho T668) phospho peptide (Lane 1) and Amyloid Precursor Protein Non-phospho peptide (Lane 2) labeled using ab206297 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 40ng/ml nocodazole for 24 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100-120 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab206297, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/1000 dilution

    All lanes : Mouse brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 86 kDa
    Observed band size: 100-120 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab206297, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Immunocytochemistry - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    This data was developed using ab206297, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Amyloid Precursor Protein (phospho T668) with ab206297 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The expression increased after treatment with nocodazole (0.04ug/ml) for 24 hours. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab206297 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    This data was developed using ab206297, the same antibody clone in a different buffer formulation.Amyloid Precursor Protein (phospho T668) was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab206297 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab206297 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab206297 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206297 in Mouse brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
    Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)

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