Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
![Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-445807-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-western-blot.jpg "Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7074(N)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free
- Suitable for: Dot blot, WB, IP, ICC
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free
See all Amyloid Precursor Protein primary antibodies -
Description
Rabbit monoclonal [EPR7074(N)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IP, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
The immunogen used for this product is within Human Amyloid Precursor Protein aa 750 to the C-terminus and therefore may detect gamma secretase fragments 50, 57 and 59 in addition to fragments C31, C80, C83 and C99. Cross-reactivity with these fragments has not been confirmed experimentally.
Ab251447 is the carrier-free version of ab206297. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251447 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR7074(N) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human hippocampus treated with Lambda Phosphatase lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 100-120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab206297, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-5-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)This data was developed using ab206297, the same antibody clone in a different buffer formulation.Dot blot analysis of Amyloid Precursor Protein (phospho T668) phospho peptide (Lane 1) and Amyloid Precursor Protein Non-phospho peptide (Lane 2) labeled using ab206297 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes. -
![Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-408292-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 40ng/ml nocodazole for 24 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 86 kDa
Observed band size: 100-120 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab206297, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-406066-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/1000 dilution
All lanes : Mouse brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 86 kDa
Observed band size: 100-120 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab206297, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Immunocytochemistry - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-2-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)This data was developed using ab206297, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Amyloid Precursor Protein (phospho T668) with ab206297 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The expression increased after treatment with nocodazole (0.04ug/ml) for 24 hours. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab206297 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-1-anti-amyloid-precursor-protein-phospho-t743-antibody-epr7074-n-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)This data was developed using ab206297, the same antibody clone in a different buffer formulation.Amyloid Precursor Protein (phospho T668) was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab206297 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab206297 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab206297 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206297 in Mouse brain whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. -
![Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)](https://www.abcam.com/ps/products/251/ab251447/Images/ab251447-6-benefits-of-recombinant-antibodies.png)
Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] - BSA and Azide free (ab251447)
