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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid

Anti-RAGE antibody [EPR16849-75] (ab181293)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-RAGE antibody [EPR16849-75] (ab181293)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16849-75] to RAGE
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-RAGE antibody [EPR16849-75]
    See all RAGE primary antibodies
  • Description

    Rabbit monoclonal [EPR16849-75] to RAGE
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat lung lysates. IP: Mouse lung whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16849-75
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Sensory System
    • Visual system
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Images

  • Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
    Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
    All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/2000 dilution

    Lane 1 : Mouse lung lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse heart lysates
    Lane 5 : Mouse liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 1 second


    RAGE expression was predominantly high in the lung reported by PMID: 21743278.

  • Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
    Western blot - Anti-RAGE antibody [EPR16849-75] (ab181293)
    All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/5000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Rat lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] (ab181293)
    Immunoprecipitation - Anti-RAGE antibody [EPR16849-75] (ab181293)

    RAGE was immunoprecipitated from 1 mg of mouse lung whole cell lysate with ab181293 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab181293 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung whole cell lysate 10µg (Input).

    Lane 2: ab181293 IP in mouse lung whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181293 in mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-RAGE antibody [EPR16849-75] (ab181293)
    Anti-RAGE antibody [EPR16849-75] (ab181293)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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