Anti-RAGE antibody [EPR16849-75] (ab181293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16849-75] to RAGE
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-RAGE antibody [EPR16849-75]
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR16849-75] to RAGE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB MouseRat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat lung lysates. IP: Mouse lung whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16849-75 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/2000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 secondRAGE expression was predominantly high in the lung reported by PMID: 21743278.
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All lanes : Anti-RAGE antibody [EPR16849-75] (ab181293) at 1/5000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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RAGE was immunoprecipitated from 1 mg of mouse lung whole cell lysate with ab181293 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab181293 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse lung whole cell lysate 10µg (Input).
Lane 2: ab181293 IP in mouse lung whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab181293 in mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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