Anti-RAGE antibody (ab54741)
Key features and details
- Mouse monoclonal to RAGE
- Suitable for: WB, IP, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-RAGE antibody
See all RAGE primary antibodies -
Description
Mouse monoclonal to RAGE -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant full length protein (GST-tag) corresponding to Human RAGE aa 23-405. Full length mature chain, without signal peptide.
Database link: Q15109 -
Positive control
- WB: Human kidney tissue lysate. RAGE transfected HEK-293T cell lysate. Recombinant RAGE protein. Flow Cytometry: Jurkat cells. IHC-P: Human lung tissue.
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General notes
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS
In 1x PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-RAGE antibody (ab54741)Anti-RAGE antibody (ab54741) at 1 µg/ml + Human kidney tissue lysate
Developed using the ECL technique.This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAGE antibody (ab54741)ab54741 (1 µg/ml) staining RAGE in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is membrane staining throughout the alveloli.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.This image was generated using the ascites version of the product.
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Western blot - Anti-RAGE antibody (ab54741)All lanes : Anti-RAGE antibody (ab54741) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) RAGE transfected cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) non-transfected cell lysate
Developed using the ECL technique.This image was generated using the ascites version of the product.
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Western blot - Anti-RAGE antibody (ab54741)Anti-RAGE antibody (ab54741) at 1 µg/ml + tagged recombinant protein immunogen
Developed using the ECL technique.Predicted band size of immunogen is 68 kDa
This image was generated using the ascites version of the product.
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Immunoprecipitation - Anti-RAGE antibody (ab54741)Immunoprecipitation of RAGE transfected lysate using ab54741 and Protein A Magnetic Bead. Immunoblotted with a rabbit polyclonal anti-RAGE antibody.
This image was generated using the ascites version of the product.
