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Anti-RAGE antibody (ab30381)

Key features and details

  • Rabbit polyclonal to RAGE
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RAGE antibody
    See all RAGE primary antibodies

  • Description

    Rabbit polyclonal to RAGE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RAGE.

    Read Abcam's proprietary immunogen policy (Peptide available as ab32414.)

  • Positive control

    • Mouse Lung Whole Tissue Lysate. T24-83 methanol fixed cell line (IF/ICC)

Properties

Applications

Our Abpromise guarantee covers the use of ab30381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.

  • Tissue specificity

    Endothelial cells.

  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Advanced glycosylation end product-specific receptor antibody
      • Ager antibody
      • DAMA 358M23.4 antibody
      • MGC2235 antibody
      • MGC22357 antibody
      • RAGE_HUMAN antibody
      • Receptor for advanced glycation end products antibody
      • Receptor for advanced glycosylation end products antibody
      see all

    Images

    • Western blot - Anti-RAGE antibody (ab30381)
      Western blot - Anti-RAGE antibody (ab30381)
      Anti-RAGE antibody (ab30381) at 1 µg/ml + Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30381 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    • Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab30381)
      Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab30381)

      ICC/IF image of ab30381 stained T24-83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab30381 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-RAGE antibody (ab30381)
      Western blot - Anti-RAGE antibody (ab30381)
      Anti-RAGE antibody (ab30381) at 1 µg/ml + Mouse Lung Whole Tissue Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?

    References (4)

    Publishing research using ab30381? Please let us know so that we can cite the reference in this datasheet.

    ab30381 has been referenced in 4 publications.

    • Hu J  et al. Protective Effects of Adiponectin Against Diabetic Renal Injury in a Mouse Model of Diabetes. Cell Physiol Biochem 43:870-878 (2017). PubMed: 28954262
    • Miller S  et al. The Ser82 RAGE Variant Affects Lung Function and Serum RAGE in Smokers and sRAGE Production In Vitro. PLoS One 11:e0164041 (2016). IHC ; Human . PubMed: 27755550
    • Zhu W  et al. Advanced glycation end products induce a prothrombotic phenotype in mice via interaction with platelet CD36. Blood 119:6136-44 (2012). WB ; Mouse . PubMed: 22431576
    • Kuhla A  et al. Oxidative stress-associated rise of hepatic protein glycation increases inflammatory liver injury in uncoupling protein-2 deficient mice. Lab Invest 90:1189-98 (2010). WB ; Mouse . PubMed: 20368701

    Images

    • Western blot - Anti-RAGE antibody (ab30381)
      Western blot - Anti-RAGE antibody (ab30381)
      Anti-RAGE antibody (ab30381) at 1 µg/ml + Mouse lung normal tissue lysate - total protein (ab29297) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab30381 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
    • Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab30381)
      Immunocytochemistry/ Immunofluorescence - Anti-RAGE antibody (ab30381)

      ICC/IF image of ab30381 stained T24-83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab30381 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-RAGE antibody (ab30381)
      Western blot - Anti-RAGE antibody (ab30381)
      Anti-RAGE antibody (ab30381) at 1 µg/ml + Mouse Lung Whole Tissue Lysate at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 47 kDa why is the actual band size different from the predicted?

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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