Anti-PGP9.5 antibody (ab227157)
Key features and details
- Rabbit polyclonal to PGP9.5
- Suitable for: WB, IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
Description
Rabbit polyclonal to PGP9.5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PGP9.5 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P09936 -
Positive control
- WB: HEK-293T, A549 and H1299 whole cell extract; rat and mouse brain tissue extract. IHC-Fr: Mouse cerebellum tissue; rat E13.5 brain tissue. IHC-P: SEROUS OVCA xenograft tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded SEROUS OVCA (human) xenograft tissue stained for PGP9.5 with ab227157 at 1/300 dilution in immunohistochemical analysis.
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Frozen section of E13.5 rat brain tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)
The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin 3 is detected with an anti-beta-Tubulin 3 antibody (red).
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All lanes : Anti-PGP9.5 antibody (ab227157) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T treated with PGP9.5 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa12% SDS-PAGE
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4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for PGP9.5 (green) using ab227157 at 1/500 dilution in ICC/IF.
The nuclear counter stain is Fluoroshield with DAPI. Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).
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Anti-PGP9.5 antibody (ab227157) at 1/10000 dilution + Rat brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa12% SDS-PAGE
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Frozen section of adult mouse cerebellum tissue stained for PGP9.5 using ab227157 at 1/250 dilution in immunohistochemical analysis (green)
The nuclear counter stain is fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-beta-Tubulin antibody (red).
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All lanes : Anti-PGP9.5 antibody (ab227157) at 1/5000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa12% SDS PAGE
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Anti-PGP9.5 antibody (ab227157) at 1/5000 dilution + Mouse brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa12 % SDS-PAGE