Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸ 10 µl 137 366 ₸

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Anti-PGP9.5 antibody [EPR4117] (ab109261)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4117] to PGP9.5
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-PGP9.5 antibody [EPR4117]
See all PGP9.5 primary antibodies
Description

Rabbit monoclonal [EPR4117] to PGP9.5
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 7.20 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4117
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)

ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)

All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Y79 lysate
Lane 3 : U-87 MG lysate
Lane 4 : SH SY5Y lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 24 kDa
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261) Image courtesy of Dr Simone Diestel by Abreview.

All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)

Lysates/proteins at 100 µg per lane.

Secondary
All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 24 kDa

Exposure time: 2 minutes
See Abreview
OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-PGP9.5 antibody [EPR4117] (ab109261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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