Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGP9.5 antibody [EPR4117] (ab109261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4117] to PGP9.5
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PGP9.5 antibody [EPR4117]
    See all PGP9.5 primary antibodies

  • Description

    Rabbit monoclonal [EPR4117] to PGP9.5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)

    ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
    Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)              

    Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Y79 lysate
    Lane 3 : U-87 MG lysate
    Lane 4 : SH SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa

  • Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261) Image courtesy of Dr Simone Diestel by Abreview.
    All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
    Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
    Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)

    Lysates/proteins at 100 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 2 minutes

    See Abreview

  • OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PGP9.5 antibody [EPR4117] (ab109261)
    Anti-PGP9.5 antibody [EPR4117] (ab109261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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