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Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)

Key features and details

  • Mouse monoclonal [PRK8] to Parkin - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Parkin antibody [PRK8] - BSA and Azide free
    See all Parkin primary antibodies

  • Description

    Mouse monoclonal [PRK8] to Parkin - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Parkin.

  • Epitope

    The epitope is the second ring domain (aa 399-465).

  • Positive control

    • WB: SH-SY5Y whole cell lysate. Human, mouse and rat brain tissue lysates. Wild-type HAP1 whole cell lysate. Flow Cyt: SH-SY5Y cells.

  • General notes

    ab264105 is a PBS only version of ab77924.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Flow Cytometry - Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)
    Flow Cytometry - Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)

    Overlay histogram showing SH-SY5Y cells stained with ab77924 (red line).

    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77924, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

    This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab77924).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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