Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)
![Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)](https://www.abcam.com/ps/products/264/ab264105/Images/ab264105-355580-anti-parkin-antibody-prk8-flow-cytometry-shsy5y-human.jpg "Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)")
Key features and details
- Mouse monoclonal [PRK8] to Parkin - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Parkin antibody [PRK8] - BSA and Azide free
See all Parkin primary antibodies -
Description
Mouse monoclonal [PRK8] to Parkin - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant full length protein corresponding to Human Parkin.
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Epitope
The epitope is the second ring domain (aa 399-465). -
Positive control
- WB: SH-SY5Y whole cell lysate. Human, mouse and rat brain tissue lysates. Wild-type HAP1 whole cell lysate. Flow Cyt: SH-SY5Y cells.
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General notes
ab264105 is a PBS only version of ab77924.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
PRK8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Parkin antibody [PRK8] - BSA and Azide free (ab264105)
Overlay histogram showing SH-SY5Y cells stained with ab77924 (red line).
The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77924, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab77924).
