Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)

Key features and details

  • Mouse monoclonal [346CT2.5.1] to PGP9.5
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PGP9.5 antibody [346CT2.5.1]
    See all PGP9.5 primary antibodies

  • Description

    Mouse monoclonal [346CT2.5.1] to PGP9.5

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human PGP9.5 aa 1 to the C-terminus.

  • Positive control

    • This antibody gave a positive signal in the following lysates: Human Brain Tissue; Rat Brain Tissue; Rat Brain Cortex Tissue; PC12 Whole Cell; Y79 Whole Cell; U-87MG Whole Cell; SHSY-5Y Whole Cell and HAP1 Wole cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    This monoclonal antibody to PGP9.5 has been knockout validated in Western blot. The expected band for PGP9.5 was observed in wild type cells and the band was not seen in knockout cells.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
    Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: UCHL1 (KO) knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab86808 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

    Ab86808 was shown to specifically react with PGP9.5/UCHL1 in wild-type cells as signal was lost in PGP9/UCHL1 knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE.  Ab86808 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/2000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
    Flow Cytometry - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
    Overlay histogram showing SH-SH5Y cells stained with ab86808 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86808, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
    Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
    All lanes : Anti-PGP9.5 antibody [346CT2.5.1] (ab86808) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 6 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 1 minute

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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