Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
![Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)](https://www.abcam.com/ps/products/86/ab86808/Images/ab86808-279569-anti-pgp95-antibody-346ct251-western-blot.jpg "Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)")
Key features and details
- Mouse monoclonal [346CT2.5.1] to PGP9.5
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PGP9.5 antibody [346CT2.5.1]
See all PGP9.5 primary antibodies -
Description
Mouse monoclonal [346CT2.5.1] to PGP9.5 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB RatHuman
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Immunogen
Recombinant full length protein corresponding to Human PGP9.5 aa 1 to the C-terminus.
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Positive control
- This antibody gave a positive signal in the following lysates: Human Brain Tissue; Rat Brain Tissue; Rat Brain Cortex Tissue; PC12 Whole Cell; Y79 Whole Cell; U-87MG Whole Cell; SHSY-5Y Whole Cell and HAP1 Wole cells.
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General notes
This antibody clone is manufactured by Abcam.
This monoclonal antibody to PGP9.5 has been knockout validated in Western blot. The expected band for PGP9.5 was observed in wild type cells and the band was not seen in knockout cells.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
346CT2.5.1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab86808 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.Ab86808 was shown to specifically react with PGP9.5/UCHL1 in wild-type cells as signal was lost in PGP9/UCHL1 knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. Ab86808 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/2000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)](https://www.abcam.com/ps/products/86/ab86808/Images/ab86808-204481-anti-pgp95-antibody-346ct251-flow-cytometry.jpg)
Flow Cytometry - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)Overlay histogram showing SH-SH5Y cells stained with ab86808 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86808, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)](https://www.abcam.com/ps/products/86/ab86808/Images/ab86808-101892-anti-pgp95-antibody-346ct251-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [346CT2.5.1] (ab86808)All lanes : Anti-PGP9.5 antibody [346CT2.5.1] (ab86808) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 6 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 1 minute
