Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNAI2 antibody [EPR11224] (ab170920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11224] to DNAI2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DNAI2 antibody [EPR11224]
    See all DNAI2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11224] to DNAI2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human DNAI2. The exact sequence is proprietary.
    Database link: Q9GZS0

  • Positive control

    • Human testis, Rat testis, Mouse testis and fetal lung lysates.

  • General notes

     This product was previously labelled as Dynein intermediate chain 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11224

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAI2 antibody [EPR11224] (ab170920)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAI2 antibody [EPR11224] (ab170920)

    Immunohistochemical analysis of paraffin embedded testis tissue labeling DNAI2 with ab170920 at 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-DNAI2 antibody [EPR11224] (ab170920)
    Immunoprecipitation - Anti-DNAI2 antibody [EPR11224] (ab170920)
    Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution + Immunoprecipitation pellet from human testis lysate using ab170920 at 1/10.

    Secondary
    Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Developed using the ECL technique.

    Additional bands at: ~50 kDa (possible IgG)

  • Western blot - Anti-DNAI2 antibody [EPR11224] (ab170920)
    Western blot - Anti-DNAI2 antibody [EPR11224] (ab170920)
    All lanes : Anti-DNAI2 antibody [EPR11224] (ab170920) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Rat testis lysate
    Lane 3 : Mouse testis lysate
    Lane 4 : Fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa

  • Anti-DNAI2 antibody [EPR11224] (ab170920)
    Anti-DNAI2 antibody [EPR11224] (ab170920)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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